271 EXPRESSION OF NADP-MALIC ENZYME RNA LEVELS IN RIPENING PEACH FRUIT
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چکیده
منابع مشابه
Limited proteolysis of maize NADP-malic enzyme.
The incubation of maize malic enzyme at 37 degrees C with trypsin at a ratio of 150:1 of malic enzyme to trypsin caused rapid and complete inactivation of enzyme activity. The inactivation was caused by fairly specific cleavage of the enzyme monomer (62 kDa) into 40 kDa and 20 kDa fragments. The intensity of 40 kDa band increased with the time of treatment of enzyme with trypsin from 2 to 30 mi...
متن کاملStructural studies of the pigeon cytosolic NADP(+)-dependent malic enzyme.
Malic enzymes are widely distributed in nature, and have important biological functions. They catalyze the oxidative decarboxylation of malate to produce pyruvate and CO(2) in the presence of divalent cations (Mg(2+), Mn(2+)). Most malic enzymes have a clear selectivity for the dinucleotide cofactor, being able to use either NAD(+) or NADP(+), but not both. Structural studies of the human mitoc...
متن کاملPrimary structure of the maize NADP-dependent malic enzyme.
Chloroplast-localized NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME) provides a key activity for the carbon 4 fixation pathway. In maize, nuclear encoded NADP-ME is synthesized in the cytoplasm as a precursor with a transit peptide that is removed upon transport into the chloroplast stroma. We present here the complete nucleotide sequence for a 2184-base pair full-length maize NADP-ME cDNA...
متن کاملPurification, cDNA cloning and heterologous expression of the human mitochondrial NADP(+)-dependent malic enzyme.
Mitochondrial NADP(+)-dependent malic enzyme (ME; EC 1.1.1.39) has been purified to homogeneity and characterized kinetically from bovine heart. Partial amino acid sequence information allowed amplification of a specific bovine cDNA, which was used to isolate a full-length human cDNA of this isoform of ME. The cDNA is 1930 bp long and codes for a protein of 604 amino acids. Comparison of the am...
متن کاملIdentification and differential expression dynamics of peach small GTPases encoding genes during fruit development and ripening
The function of monomeric GTPases of the RAS superfamily in fruit development and ripening has been partially characterized. Here the identification of peach (Prunus persica) small GTPases of the RAS superfamily expressed in fruit and the characterization of their expression profiles during fruit development are described. Extensive searches on expressed sequence tag (EST) databases led to the ...
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ژورنال
عنوان ژورنال: HortScience
سال: 1994
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.29.5.468f